News

Page / Total Page 1
The University Research Facility in Life Sciences (ULS) is co-organizing an online seminar on the Surface Plasmon Resonance (SPR) System with Cytiva. SPR is a label-free spectroscopic technique for studying molecular interactions (binding affinities, association/dissociation kinetics) in real-time using relatively small quantities of materials (organic compounds, nucleic acid, glycoproteins, viruses, etc.). This technology is not only valuable in the fundamental understanding of biological processes but also in drug discovery. For more information about the technology, please visit the website of Cytiva.

The details of the upcoming seminar are as follow:
· Date: 8th October 2021 (Friday)
· Time: 10:00 – 11:30 am
· Speaker: Michael Luo, Advanced Product Specialist, Cytiva
· Rundown: (1) Introduction on the Biacore SPR system; (2) Application on studying molecular interaction of protein, organic compounds, nanoparticles, etc, for the understanding of biological processes and drug screening

If you are interested in attending this online seminar, please complete the online registration form by 7th October 2021. Successful registrants will receive a confirmation email and the Microsoft Team meeting link one working day before the event. For any inquires on the seminar, please contact Dr. Carol Tsang.
Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the Cytiva AKTA pure 25 M1 for purification of proteins, peptides, and nucleic acids is now available for booking. The system allows flexibility in purification of proteins, peptides and nucleic acids by different chromatography techniques, including ion exchange chromatography, affinity chromatography and gel exclusion chromatography, etc. Please refer to the following link for details and charges of the instrument and contact Dr Ryan Chow for any enquiries on its applications and for arranging a training.

Thank you for your attention.
 
Mass cytometry, or Cytometry by Time-Of-Flight (CyTOF), is a powerful platform for high-dimensional profiling of cell phenotypes and signalling pathways at single cell resolution.

In this seminar, the technology of Mass Cytometry for cells in suspension and Imaging Mass Cytometry for Cellular Tissues, the workflow and its capabilities in the context of deep immune profiling and applications into tissue immune microenvironment will be discussed.

Title: Igniting New Insights with CyTOF® - with Highly Multiplex Mass Cytometry and Imaging Mass Cytometry
Date: 28 July 2021 (Wednesday)
Time: 2:30pm - 4:00pm
Speaker: Dr. TEH Boon-Eng (Senior Manager - Service and Support; Fluidigm Greater Asia)
Venue: Online via ZOOM

Please click HERE for registration (Link for online seminar will be sent to registered users by email later)
Should you have any enquiries, please contact Dr. Rachel Li (email: rachel.ws.li@polyu.edu.hk).
Thank you for your attention.
 
The fifth issue of the ULS newsletter has been issued. Please follow the link to view it online.

The ULS newsletter aims at introducing the latest technologies and innovations in life science, promoting new and existing equipment managed by the ULS, as well as encouraging users and principal investigators to connect with us. We welcome readers to give us comments and suggestions as we strive to make our contents timely, relevant and interesting.

Thank you very much for your support.
 
We are glad to announce that the 5-lasers FACSAriaIII cell sorter in ULS has recently been upgraded with an Automatic Cell Deposition Unit (ACDU) which users can perform single cell sorting or index sorting into 6, 24, 48, 96, and 384-well plates. It is an important additional tool for subsequent single cell analysis. For more information or training session of this accessory, please do not hesitate to contact Dr. Ryan Chow (34008057) or Dr. Rachel Li (34008056) for arrangement. Thank you very much for your attention.
 
The reports of the ULS Survey 2020 have been released and can be viewed from the Download area of the ULS webpage. On the other hand, 20 survey participants were randomly selected to each receive 2 hours of free equipment usage for their laboratories in March and the result has been announced on the ULS Facebook Page.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the TECAN Automated Liquid Handling System (Freedom EVO 100, 150 and 200) for Drug Screening and Protein Purification is now available for booking. Please refer to the following link for details and charges of the instrument: HERE, and contact Dr Carol Tsang (Email: carol.cs.tsang@polyu.edu.hk; ext.: 8980) for any enquiries on its applications and for arranging a training. Current users should contact Dr Carol Tsang to arrange for a refresher training in order to renew their access to the instrument.

Thank you for your attention.
 
The ULS survey for 2020 has commenced and all current ULS users and PIs would receive an email with details of the survey. Your participation is of great importance for the ULS to understand our strengths and shortcomings. All survey participants will enter into a lucky draw and a total of 20 winners will each receive 2 hours of free ULS equipment usage for their laboratories in March. Please complete the survey by 10 February and contact us if you have any queries.
 
You are cordially invited to attend the ULS online seminar. Details are as follows:

Title: Multi-color & Single Cell Flow Analysis
Date: 16 Dec 2020 (Wednesday)
Time: 2:00pm – 3:30pm
Speaker:
Ms. Ivy Chen (BD Biosciences)
• How to design Panel: rules & myths
• Multicolor Instrument Setup in FACSDiva
• Tools available for panel design & analysis

Dr. Yin Lui (Bio-Gene Technology Ltd.)
• The solution of Single Cell Sorting & Multi-Omics Analysis: BD Melody & Rhapsody

Venue: Online via Microsoft Teams
Registration link: HERE (Link for online seminar will be sent to registered users by email later)

Should you have any enquiries, please contact Dr. Ryan Chow (email: bcchy@polyu.edu.hk)
Thank you for your attention.
 
You are cordially invited to attend the ULS online seminar. Details are as follows:

Title: Rapid functional evaluation of cell growth and viability using multi-parametric live cell analysis

Date: 10 Dec 2020 (Thursday)
Time: 11:00 a.m.- 12:00 noon
Speaker: Ms. Stephanie Ting (Field Application Scientist, Agilent Technologies Inc.)
Venue: Online via Webex
Registration link: HERE (Link for online seminar will be sent to registered users by email later)

Should you have any enquiries, please contact Dr. Rachel Li (email: rachel.ws.li@polyu.edu.hk)
Thank you for your attention.
 
You are cordially invited to attend the ULS online seminar. Details are as follows:

Title: New Advances in Live-Cell Imaging and Analysis

Date: 4 December 2020 (Friday)
Time: 3:30pm - 4:30pm
Speaker: Jenny Li (Field Application Scientist Area Manger, Sartorius (Shanghai) Trading Co. Ltd)
Venue: Online via Teams

Registration link: HERE (Link for online seminar will be sent to registered users by email later)

Should you have any enquiries, please contact Dr. Rachel Li (email: rachel.ws.li@polyu.edu.hk).
Thank you for your attention.
 
The fourth issue of the ULS newsletter has been issued. Please follow the link to view it online.

The ULS newsletter aims at introducing the latest technologies and innovations in life science, promoting new and existing equipment managed by the ULS, as well as encouraging users and principal investigators to connect with us. We welcome readers to give us comments and suggestions as we strive to make our contents timely, relevant and interesting.

Thank you very much for your support.
 
The FUJIFILM Vevo LAB software has now been moved to the PolyU Universal Desktop Services (UDS), which is a remote desktop sharing platform where user can access without time and location constraint. All ULS Vevo LAZR users are now eligible to book the software via the ULS booking system and access the software through the ULS UDS platform with this link. Please be reminded that booking is necessary in order to use the software, and the maximum usage time is 2 hours per session. The offline Vevo Lab software at Y708 is suspended with immediate effect.
 
The 2 licensed flow cytometry softwares, FlowJo and Modfit LT, have now been moved to the PolyU desktop sharing platform called Universal Desktop Services (UDS), which is a remote desktop sharing platform where user can access without time and location constraint. All ULS flow cytometer users are now eligible to book the software via the ULS booking system and access the software through the ULS UDS platform with this link. Please be reminded that booking is necessary in order to use the software, and the maximum usage time is 2 hours per session. The offline flowing analysis platform at Y709 is suspended with immediate effect.
 
In view of the recent resurgence of Covid-19 cases, the ULS has temporarily suspended all face-to-face equipment training with immediate effect. While access to ULS equipment and technical support for current users are unaffected, all personnel are strictly required to properly wear a face mask at all times inside any ULS laboratory. The ULS will be closely monitoring the situation and will notify users when there are updates on the arrangement. If you have any queries, please do not hesitate to contact our staff. Thank you for your attention.
 
Laser line of FACSAria III is upgraded from 3 lasers to 5 lasers. Maximum 12 fluorescence signals can be detected at the same time. Please read the equipment description page for more information.
 
The third issue of the ULS newsletter has been issued. Please follow the link to view it online.

The ULS newsletter aims at introducing the latest technologies and innovations in life science, promoting new and existing equipment managed by the ULS, as well as encouraging users and principal investigators to connect with us. We welcome readers to give us comments and suggestions as we strive to make our contents timely, relevant and interesting.

Thank you very much for your support.
 
The ULS website is scheduled for maintenance on 21 May 2020 from 9:00 to 13:00. All functions on ULS website will not be available during the maintenance period. We are sorry for any inconvenience caused.
 
As the University has resumed normal operation this week, the ULS will be resuming equipment training with immediate effect. Please contact our staff for enquiry or arrangement.
 
The University Research Facility in Life Sciences (ULS) is now collecting publication and research grant information involving ULS equipment or services. PIs would receive by email a unique URL to fill in an online form. We hope you could spare a few minutes to provide us with the required information by 24th April, 2020. Credits for equipment usage will be awarded upon receipt of such information (if that had not been awarded before). For journal publications, credits for 3 hours of equipment usage will be awarded; for conference papers, theses and other publications, credits for 1 hour of equipment usage will be awarded.
 
The ULS survey for 2019 has commenced and all current ULS users and PIs would receive an email with details of the survey. Your participation is of great importance for the ULS to understand our strengths and shortcomings. All survey participants will enter into a lucky draw and a total of 20 winners will each receive 2 hours of free ULS equipment usage for their laboratories in May, 2020. Please complete the survey by 17 April and contact us if you have any queries.
 
In view of the current outbreak of the Novel Coronavirus (2019-nCoV), the ULS will be temporarily suspending all face-to-face equipment training with immediate effect. While access to ULS equipment and technical support for current users are unaffected, all personnel are strictly required to properly wear a face mask at all times inside any ULS laboratory. The ULS will be closely monitoring the epidemic and will notify users when there are updates on the arrangement. If you have any queries, please do not hesitate to contact our staff. Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) will be updating our Equipment Charging Scheme with effect from 1 February, 2020. In the meantime, we are pleased to offer discounts to internal research groups with monthly usage exceeding HK$5000 (10% discount) or HK$10000 (20% discount). If you have any queries about our new charging scheme, please do not hesitate to contact our staff.
 
The University Research Facility in Life Sciences (ULS) is co-organising a seminar on Stimulated Emission Depletion (STED) Nanoscopy with Leica Microsystems, Inc. STED is a confocal-based super-resolution fluorescence microscopy technique that is known for its ease of use and minimal image artefacts. It delivers a lateral resolution of up to 50 nm. Please visit the Leica website for more information about the technology, and see below for details of the upcoming seminar:

Date: 10th October, 2019 (Thursday)
Time: 3:00 to 4:00 pm
Venue: Y715, Lee Shau Kee Building (Block Y)
Speaker: Bob Tang, Applications Specialist, Leica Microsystems, Inc.

Registration is not required for the seminar, but seating will be on a first-come-first-served basis. Please contact Dr Michael Yuen if you have any queries.
 
The second issue of the ULS newsletter has been issued. Please follow the link to view it online.

The ULS newsletter aims at introducing the latest technologies and innovations in life science, promoting new and existing equipment managed by the ULS, as well as encouraging users and principal investigators to connect with us. We welcome readers to give us comments and suggestions as we strive to make our contents timely, relevant and interesting.

Thank you very much for your support.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce a major upgrade of the Nikon N-SIM/N-STORM/A1 Super-resolution/Confocal Microscope. In addition to featuring two super-resolution techniques (i.e., SIM and STORM) in one single system, the system is now equipped with a confocal scan head for performing laser scanning confocal microscopy, thus enabling researchers to perform correlative confocal-super-resolution imaging.

Please refer to the ULS webpage for further details of the equipment, as well as for a sample image acquired in-house. For any enquiries on the equipment and for arranging for a training, please contact Dr Michael Yuen.
 
The Countess II FL Automatic Cell Counting and Imaging System will be available for booking in mid-July. The new system is capable of acquiring bright-field and fluorescence images of cell suspensions; and by automatic analysis of the images, allow researchers to instantaneously obtain such information as the cell count, labelling of fluorescent probes, expression of fluorescent proteins and cell viability, etc. Two training sessions have been arranged and the details are as follows:

- Date: 16th July, 2019 (Tuesday)
- Time: 3:30 to 4:15 pm (session 1); 4:30 to 5:15 pm (session 2)
- Venue: Y709, Lee Shau Kee Building

If you would like to sign up for the training, please email Dr Michael Yuen and provide your name, department, contact number and preferred session. Registrants will receive a confirmation email no later than 10th July.
 
The University Research Facility in Life Sciences (ULS) is hosting a seminar on the ProteinSimple Capillary Western Blotting System. The details are as follows:

Date: 5th July, 2019 (Friday)
Time: 11:00 to 12:00
Venue: Y716, Lee Shau Kee Building (Block Y)

Registration is not required for the seminar and demo, but seating will be on a first-come-first-served basis. Please contact Dr Michael Yuen if you have any queries.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the Logos Biosystems X-CLARITY Tissue Clearing System is now available for booking. The new system comprises a polymerisation system for creating tissue-hydrogel hybrid constructs, and an electrophoretic tissue clearing system to remove lipids in the samples, making the latter largely transparent and thus suitable for 3-dimensional, deep-tissue imaging (e.g., using multiphoton or light sheet fluorescence microscopy). Please refer to Chung et al. (Nature 497, 332-337 (2013)) or the ULS webpage for further details of the technique, and contact Dr Michael Yuen for any enquiries on its applications and for arranging for a training.
 
The University Research Facility in Life Sciences (ULS) has recently upgraded our Imaris 3/4D image visualisation and analysis software to the latest version. In addition to 3D volumetric rendering and 3D colocalisation analysis, the version 9.3 now features image deconvolution, a commonly used image processing technique to improve both the resolution and contrast of images. For more details, please refer to the Imaris website or to sample images acquired in-house by the ULS.

Please contact Dr Michael Yuen if you have any queries or if you would like to arrange for a training. Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the JASCO CPL-300 Circularly Polarized Luminescence (CPL) Spectrophotometer is now available for booking. For details and charges of the instrument, and recent examples for its applications, please check the following link: https://uls.polyu.edu.hk/Equipment/Index.

Please contact Dr Carol Tsang (Email: carol.cs.tsang@polyu.edu.hk; Ext.: 8980) for any enquires on the application and training.
 
A training workshop for the JASCO CPL-300 Circularly Polarized Luminescence (CPL) spectrometer will be held on 25th April, 2019 in Y709. Two sessions are available for booking:

Morning session : 10:00 am to 12:00 pm
Afternoon session: 2:30 pm to 4:30 pm

Due to space limitation, attendance of the workshop will be on a first-come, first-served basis. Please email Dr. Carol Tsang (carol.cs.tsang@polyu.edu.hk) with your contact information and preferred session for enrolment or for enquiry. For details of the instrument, please check the following link: https://jascoinc.com/products/spectroscopy/circularly-polarized-luminescence-cpl-300/. For the fundamental and application of CPL, please check the following link: https://onlinelibrary.wiley.com/doi/full/10.1002/chir.22647.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the Nikon SMZ1270i Fluorescence Stereomicroscope is now available for booking. The new system is a parallel-optics and zooming type stereomicroscope offering advanced operability, as well as high magnification, zoom ratio and resolution. Please refer to the following link for details and charges of the instrument: https://uls.polyu.edu.hk/Equipment/Index, and contact Dr Michael Yuen for any enquires on its applications and for arranging for a training.
 
The University Research Facility in Life Sciences (ULS) is co-organising a seminar and a demonstration on the ImageXpress Pico Automated Cell Imaging System with Molecular Devices. The details are as follows:

Date: 23rd April, 2019 (Tuesday)
Time: 2:00 to 2:30 pm (seminar); 2:30 to 3:30 pm (demo)
Venue: Y716, Lee Shau Kee Building (Block Y)
Speaker: Dr Ting Fung Tsang, Product Specialist, Molecular Devices

Registration is not required for the seminar and demo, but seating will be on a first-come-first-served basis. If you would like to try the system using your own sample, please contact Dr Michael Yuen by 18th April for arrangement.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the Nikon Ti2-E Live-cell Fluorescence Imaging System is now available for booking. The new system is an inverted, fully motorised, large-field-of-view wide-field fluorescence microscope equipped with a collection of state-of-the-art features for live-cell and large-tissue tile imaging. Please refer to the following link for details and charges of the instrument: https://uls.polyu.edu.hk/Equipment/Index, and contact Dr Michael Yuen for any enquires on its applications and for arranging for a training.
 
The University Research Facility in Life Sciences (ULS) is co-organising the Antibody Day 2019 with Thermo Fisher Scientific. Details are as follows:

Date: 14th March, 2019 (Thursday)
Time: 10:30 a.m. to 5:30 p.m.
Venue: Y716, Lee Shau Kee Building (Block Y)

There will be up-to-date information about antibodies and on-site technical support on imaging/flow cytometer/HCS. Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the JASCO J-1500 CD spectrometer is now available for booking. Please refer to the following link for details and charges of the instrument: https://uls.polyu.edu.hk/Equipment/Index, and contact Dr. Carol Tsang (Email: carol.cs.tsang@polyu.edu.hk; Ext.: 8980) for any enquires on its applications and for arranging a training.
 
A training workshop for the JASCO J-1500 Circular Dichroism (CD) Spectrometer will be held next Wednesday (27th February, 2019) at 14:30 in Y709. Due to space limitation, attendance of the workshop will be on a first-come, first-served basis. Please email Dr Carol Tsang (carol.cs.tsang@polyu.edu.hk) with your contact information for enrolment or enquiry. For details of the instrument, please check the following link: https://jascoinc.com/products/spectroscopy/circular-dichroism/j-1000-series-models/j-1500-circular-dichroism-spectrophotometer/.
 
The first issue of the ULS newsletter has been issued and can be viewed online.

The ULS newsletter aims at introducing the latest technologies and innovations in life science, promoting new and existing equipment managed by the ULS, as well as encouraging users and principal investigators to connect with us. We welcome readers to give us comments and suggestions as we strive to make our contents timely, relevant and interesting.
 
The University Research Facility in Life Sciences (ULS) will be updating our Equipment Charging Scheme with effect from 1 January, 2019. In the meantime, we are pleased to offer discounts to internal research groups with monthly usage exceeding HK$5000 (10% discount) or HK$10000 (20% discount). If you have any queries about our new charging scheme, please do not hesitate to contact our staff.
 
The ULS is now offering 20 sets of 96-well plate with sealing foil at NO COST! To get this offer, simply make a booking of LC480 via the ULS online booking system and forward the booking confirmation email to Dr. Ryan Chow. Ryan will contact you to get the 96-well plate with seal on the date (or the date before) of usage. Act now, the promotion is on a First-Come-First-Served basis. For more real-time PCR technical information, please do not hesitate to contact Ryan.
 
You are cordially invited to a technology seminar on how shear stress caused by liquid flow affects cell morphology and behaviour.

For more details of the technology, please refer to the following links:
https://ibidi.com/content/303-why-cell-culture-under-flow
https://ibidi.com/content/139-mv-10

Details of the seminar are as follows:
Date: Oct 29, 2018 (Monday)
Time: 3:00 - 4:00pm
Venue: Y716, Lee Shau Kee Building (Block Y)
The Hong Kong Polytechnic University
Speaker: Dr. Christian Leibold (Director Global Sales, ibidi GmbH)

Online registration: Click here

For enquiries, please contact Clara Hung (clara.hung@polyu.edu.hk; Tel: 3400 8056)
All are welcome!
 
The University Research Facility in Life Sciences (ULS) is co-organising a seminar on holotomographic microscopy with Chinetek Scientific. Details are as follows:

• Date: 30th August, 2018 (Thursday)
• Time: 2:30 to 3:30 pm
• Venue: Y817, Lee Shau Kee Building (Block Y)
• Speaker: Dr. Eliot Park (VP of Tomocube)

Tomocube’s holotomography series utilizes optical diffraction tomography (ODT) to enable users to quantitatively and noninvasively investigate biological cells and thin tissues. ODT can be used to reconstruct live cells to provide structural and chemical information about the cell, including dry mass, morphology, as well as dynamics of the cellular membrane.

Registration is not required for this seminar, but seating will be on a first-come-first-served basis. Please contact Dr Michael Yuen if you have any queries about the seminar. Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) is co-organising a "Service Day" with Thermo Fisher Scientific. Details are as follows:

• Date: 22nd August, 2018 (Wednesday)
• Time: 10:30 am to 4:30 pm
• Venue: Y715-Y716, Lee Shau Kee Building (Block Y)

There will be workshops on the operation and maintenance of freezers, CO2 incubators and centrifuges, as well as free functional check for these instrument (the latter requires prior registration). Please refer to the attached poster for the rundown of the Service Day, and register via https://goo.gl/forms/fpJXSL5Mt4wD77vt1.

Please contact Dr Michael Yuen if you have any queries. Thank you for your attention.
 
The University Research Facility in Life Sciences (ULS) is organizing a seminar on “Total Solution for Chiral Analysis” presented by Mr. Derek Hung who is the sales manager from Sunway Scientific Corp. Details are as follows:

• Date: 26th July 2018 (Thursday)
• Time: 14:00–15:00
• Venue: Y715, Lee Shau Kee Building (Block Y)

In the seminar, theory and instrumentation of different analytical techniques for chiral analysis including circular dichroism (CD), circularly polarized luminescence (CPL) and vibrational circular dichroism (VCD) will be introduced. Application examples in biological and material science will also be given, which can help users to get familiar with the capabilities of these techniques.

For any enquires on the seminar, please contact Dr. Carol Tsang (Email: carol.cs.tsang@polyu.edu.hk; Tel: 3400 8057).
 
The University Research Facility in Life Sciences (ULS) is holding a demonstration of the Nikon Eclipse Ti2 live cell imaging system. The main features of the Ti2 system include:

• Motorised stage – semi-automatic image stitching for large tissue sections, multi-well plate acquisition, 3D image reconstruction
• 25-mm field of view (FOV) – enhancing efficiency by 2 times
• Dual cameras –imaging of faint fluorescent signals and true colours
• Perfect focus system to offset fluctuations in axial focus during live-cell imaging
• Real-time deconvolution and deblurring
• High-content screening and batch analysis software module

Three identitical demonstration sessions have been arranged on 17 July 2018 (Tue) in Y708a, Lee Shau Kee Building (Core Y):

• Session 1: 10:30 to 11:30
• Session 2: 14:00 to 15:00
• Session 3: 16:00 to 17:00

If you are interested in the demonstration, please email Dr Michael Yuen (michael.yf.yuen@polyu.edu.hk) and provide your name, department, contact number and preferred session. Registrants will receive a confirmation email no later than 16 July 2018.
 
You are cordially invited to a training workshop on the applications of the Seahorse XFe24 extracellular flux analyzer.

Details of the workshop are as follows:
Date: April 19, 2018 (Thursday)
Time:
09:30 am - 10:30 am: Introduction on Seahorse XF technology
10:30 am - 5:00 pm: Training Workshop
(i) Hands-on practical session
(ii) Key to success for XF assays
(iii) Data analysis and Troubleshooting
(iv) Discussion on advanced experiments
Venue: Y715, Lee Shau Kee Building (Core Y), The Hong Kong Polytechnic University
Speaker: Dr. Edmund Tung (Assistant Technical Manager, Bio-Gene Technology Ltd.)

**Registration is required**
Please register via the following link: Registration

For enquiries, please contact Clara Hung (clara.hung@polyu.edu.hk / 3400 8056) or Ryan Chow (hoyin.chow@polyu.edu.hk / 3400 8057).

All are welcome!
 
The University Research Facility in Life Sciences (ULS) is pleased to announce that the Waters ACQUITY UPLC H-Class System is now available for online booking. It is a high resolution LC system which supports both HPLC and UPLC methods to improve the efficiency in scaling from HPLC to UPLC. For more details and specifications of the equipment, please check through the ULS website.
You are now cordially invited to a training session on the Waters ACQUITY UPLC H-Class System. Details are as follows:

Date and time: 16/4/2018 (10:30-11:30); 16/4/2018 (14:30-15:30); 18/4/2018 (10:30-11:30) or 18/4/2018 (14:30-15:30); 4 sessions are identical, please attend either one
Venue: Y729

Please register via the following link: Registration
For enquiry, please contact Dr. Carol Tsang (carol.cs.tsang@polyu.edu.hk) or Dr. Ryan Chow (bcchy@polyu.edu.hk).

All are welcome!
 
You are cordially invited to a seminar on the applications of the Sable Promethion’s Metabolic Systems.
Details of the seminar are as follows:
Title: Using behavioral data to supplement and enrich traditional metabolic phenotyping: Some case studies
Date: March 28, 2018 (Wednesday)
Time: 10:30 - 11:30 am
Venue: Y715, Lee Shau Kee Building (Core Y), The Hong Kong Polytechnic University
Speaker: John Lighton, Ph.D.
(President & CTO, Sable Systems International)

Please register via the following link: Registration
For enquiries, please contact Rachel Li (rachel.ws.li@polyu.edu.hk / 3400 8056).

All are welcome!
 
ULS is co-organizing a joint technology seminar with Qiagen on the advanced RNA technology for Next-Generation Sequencing (NGS) and bioinformatics.

Details of the seminar are as follows:

Title: Sample to Insight: Advanced RNA Technology for Next-Generation Sequencing (NGS) and Bioinformatics
Date: March 21, 2018 (Wednesday)
Time: 14:30 pm – 16:00 am
Venue: Y715, 7/F, Lee Shau Kee Building (Block Y), The Hong Kong Polytechnic University
Speaker: Mr. Kyle Wang (Market Development Manager, Marketing LS & AT) and Mr. Henry Wang (Key Account Manager, Marketing & Market Development APEC)
Abstract:
Understanding the RNA universe within a biological specimen is key to unlock gene expression and regulation mechanisms. However, RNA is a challenging analyte which requires the researcher to take special care at every step of the workflow from sample collection to results interpretation to not introduce any bias and obtain accurate results. This seminar will guide through the different “Sample to Insight” workflow steps from sample preparation to analysis. A special focus will be given to NGS technology and comprehensive bioinformatics tools for the journey of RNA discovery. RNA library prep kits for different types of RNA, targeted enrichment panels for scanning RNA from different types of samples, and the comprehensive Ingenuity Pathway Analysis (IPA) software will be highlighted for uncover data derived from ‘omics experiments, such as RNA-seq, small RNA-seq, microarrays including miRNA and SNP, metabolomics, proteomics, and small scale experiments.
For enquiries, please contact Ryan Chow (hoyin.chow@polyu.edu.hk or 3400 8057).
All are welcome!
 
The General User Policy of the ULS has been revised. Users and supervisors shall pay special attention to the new section 7, which lays out the updated equipment-sepcific safety policies and access arrangements.
 
The University Research Facility in Life Sciences (ULS) is holding a demonstration of the Leica DMi8 widefield and live cell imaging platform. The main features of the DMi8 system include:

• Motorised stage – semi-automatic image stitching for large tissue sections, multi-well plate acquisition, 3D image reconstruction
• DFC9000 GTC sCMOS camera – fast live cell imaging (90 fps at 2048×2048 resolution)
• Adaptive focus control – maintaining the focus during time-lapse imaging
• Multiple contrasting techniques – brightfield, phase contrast, differential interference contrast (DIC), polarised light

If you are interested in the demonostration, please email Dr Michael Yuen (michael.yf.yuen@polyu.edu.hk) and provide your name, department, contact number and preferred session. Registrants will receive a confirmation email no later than 12 Dec 2017.
 
ULS is co-organizing a joint technology seminar with Agena Bioscience on the use of MassArray EpiTyper for quantitative DNA methylation analysis.

Details of the seminar are as follows:
Title: Introduction to MassArray EpiTyper application of Agena Bioscience on quantitative DNA methylation analysis
Date: December 7, 2017 (Thursday)
Time: 10:00 am – 11:00 am
Venue: Y719, 7/F, Lee Shau Kee Building (Block Y), The Hong Kong Polytechnic University
Speaker: Dr. Johannes Chi-yun CHING, PhD, Senior Scientist, Agena Bioscience

For enquiries, please contact Ryan Chow (hoyin.chow@polyu.edu.hk or 3400 8057).

All are welcome!
Click here for poster
 
 
 
ULS is co-organizing a joint technology seminar with Qiagen on the use of single-stranded LNA™-enhanced antisense oligonucleotides to knockdown RNA targets in vitro.

Details of the seminar are as follows:
Title: The evaluation of long non-coding RNA targets by antisense GapmeRs and the functional study of microRNA biomarkers by inhibitors and mimics
Date: November 28, 2017 (Tuesday)
Time: 10:30 am – 11:30 am
Venue: Y716, 7/F, Lee Shau Kee Building (Block Y), The Hong Kong Polytechnic University
Speaker: Dr. Michael Hansen (Exiqon Senior Scientist and Technical Specialist)
Abstract:
The single stranded LNA™-enhanced antisense oligonucleotides (ASOs, also known as LNA™ GapmeR), which catalyze RNase H dependent degradation of both mRNAs and lncRNA, have been demonstrated to show potent knockdown of multiple classes of RNA target in vitro irrespective of the type of RNA target and its subcellular localization. In an in vivo animal model, we further report highly efficient and long lasting knockdown of a nuclear retained lncRNA in a broad range of tissues in mice subjected to systemic administration of a LNA™ GapmeR. To design LNA™ GapmeRs, we have developed an empirically derived design algorithm to provide ASOs that achieve potent target knockdown with a high hit-rate. Recent publication and our own preliminary results indicate that unspliced primary transcripts are in fact the true targets of GapmeRs and therefore the design of LNA™ GapmeRs targeting introns is a valid and efficient approach to prevent production of mature spliced transcripts. However, these results have further implications for the design to avoid off-targets, which are a relevant concern for all antisense strategies and as well toxicity. To address potential off targets located in either exons or introns, our LNA™ GapmeR design algorithm searches both spliced and unspliced transcriptomes in the Ensembl database, to provide maximal target specificity.
The functional analysis of LNA™ GapmeR in vitro and in vivo will be presented. Together with microRNA mimics and inhibitors and target site blockers, these LNA containing ASOs offer a unique set of tools for functional analysis of transcriptional regulation in human and other mammalian systems

For enquiries, please contact Ryan Chow (hoyin.chow@polyu.edu.hk or 3400 8057).

All are welcome!
Click here for poster
 
 
 
ABCT and ULS is co-organizing a joint seminar with Dr Haim M. Barr from the Weizmann Institute of Science, Israel. Details are as follows:

Date: 27th October, 2017 (Friday)
Time: 2:30 to 3:30 pm
Venue: Y817, Lee Shau Kee Building (Block Y)
Speaker: Dr Haim M. Barr, Head, Maurice and Vivienne Wohl Institute for Drug Discovery, Weizmann Institute of Science, Israel

Kindly refer to the attached poster for the abstract of the seminar and the biography of Dr Barr. Click here for poster
 
ULS is co-organizing a technology seminar with Pall Fortebio and Bio-Gene Technology Ltd. on the principle and applications of Bio-Layer Interferometry (BLI).

BLI has emerged as the leading technology for label-free and real-time biomolecular interaction analysis (BIA). Unlike traditional BIA technologies such as SPR and ITC that are time-consuming and low in throughput, BLI’s microfluidics-free, microplate based sample analysis method allows fast and accurate binding affinity and kinetics (KD, ka and kd) measurements in challenging sample matrices such as culture supernatants and lysates. The Dip and Read™ nature of BLI enabled high-throughput biophysical analyses of interactions between a wide range of biomolecules including proteins, peptides, nucleic acids, small molecules and large complexes such as viruses and liposomes. BLI data is frequently published in top scientific journals, appearing in over 2000 papers in the last 10 years. In this presentation, we will discuss about how BLI has accelerated our users’ research in:
• Antibody discovery & development
• Protein structure and function
• DNA-binding proteins
• Infectious diseases
• Small molecule drug discovery

Details of the seminar are as follows:
Title: Bio-Layer Interferometry: A leading label-free technology for biomolecular interaction analyses and high throughput screening
Date: October 25, 2017 (Wednesday)
Time: 11:00 am
Venue: Y715, Lee Shau Kee Building (Core Y), The Hong Kong Polytechnic University
Speaker: Mr. Terence Hui (Field Application Scientist Manager USA, Pall Fortebio)

For enquiries, please contact Clara Hung (clara.hung@polyu.edu.hk or 3400 8056).
All are welcome!
 
 
 
 
 
 
The ULS is co-organising a seminar with Andor Technology Limited seminar about the Andor Dragonfly high-speed confocal imaging system.

Details of the seminar are as follows:
Date: 20th September, 2017 (Wednesday)
Time: 3:00 to 4:00 pm
Venue: Y716, Lee Shau Kee Building (Core Y)
Speaker: Mr. Jacky Xiaowei Deng, Head of Sales (China), Andor Technology Limited

Abstract:
High Speed Confocal Microscope System - Andor Dragonfly It’s more than Confocal. The Andor Dragonfly allows you to image at unrivalled speed and sensitivity, meanwhile, still gentle enough for live cells. Employing a multi-point confocal system, the Dragonfly can capture at speeds at least 10x faster than conventional confocal technology, making it the optimal solution for live cell imaging or fast volume acquisition of thicker samples. Designed with patented perfect illumination delivery technology, Borealis, exceptionally flat and uniform illumination across a large field of view allows the Dragonfly to use of the newest large sensor sCMOS and EMCCD cameras to satisfy high-throughput imaging needs.
 
Light sheet fluorescence microscopy (LSFM) is a novel imaging technique that allows researchers to obtain optical sections of large biological samples with virtually no phototoxicity at unprecedented speed. The Zeiss Lightsheet Z.1 system which is available at the University Research Facility in Life Sciences (ULS) will be open for online booking in October. The ULS is organising two identical workshops to demonstrate the sample preparation procedure and imaging using zebrafish larva, and to allow users to gain hands-on experience with LSFM. The details of the workshops are as follows:

• Date: 22nd September, 2017 (Friday)
• Time: 10 am to noon (session 1) and 2:30 to 4:30 pm (session 2)
• Venue: Y405, Lee Shau Kee Building (Core Y)

If you are interested in the workshop, please email Dr. Michael Yuen and provide your name, department, contact number and preferred session. You are also welcome to contact Michael if you would like to discuss whether LSFM is suitable for your research.
 
ULS is co-organising a two-day training program on the applications of isothermal titration calorimetry (ITC) with Malvern Instruments Ltd. It will discuss recent advanced ITC applications and share experiences on using ITC in different research areas.

Details of the training workshop are as follows:
Day 1: Technology Seminar and Training Workshop
Title: How to Use Isothermal Titration Calorimetry (ITC) Better?
Date: September 26, 2017 (Tuesday)
Time: 9:30 am - 5:30 pm
Venue: Y716, Lee Shau Kee Building (Core Y), The Hong Kong Polytechnic University
Speaker: Dr. Peiwei Han (Bioscience Business Development Manager, Malvern Instruments Ltd)

Day 2: Hands-on Training Session
Date: September 27, 2017 (Wednesday)
Time: Session 1 (9:30 am - 12:30 pm)
Session 2 (2:30 pm – 5:30pm)
Venue: Y729, Lee Shau Kee Building (Core Y), The Hong Kong Polytechnic University
Speaker: Mr. Tim Han (Service Engineer, Malvern Instruments Ltd)

*Registration is required for attending the training workshop. Please register via the following link by Sep 25, 2017:
https://www.polyu.edu.hk/mysurvey/index.php/472751
 
The ULS is co-organising a seminar with Agena Bioscience to introduce the Agena MassArray nucleotide mass spectrometer and its applications. It will focus on the basic principles, applications and data analysis for the MassArray Analyzer 4 system in the ULS. Details of the seminar are as follows:

Title: Introduction to Agena MassArray Nucleotide Mass Spectrometer and its Applications
Date: 14th July, 2017 (Friday)
Time: 2:30 pm - 4:00 pm
Venue: Room Y715, Core Y
 
The revised charging scheme will take effect on 1st July, 2017. Please contact the equipment-in-charge if you have any queries.
 
The following phase 2 equipment will be available for online booking from 1st July, 2017:
  • Agena Bioscience MassARRAY Analyzer 4 System
  • Roche LightCycler 480 II Real-time PCR System
  • Applied Biosystems QuantStudio 7 Flex Real-time PCR System
  • Zeiss Lightsheet Z.1 Microscope
  • Perkin-Elmer IVIS Lumina Series III Pre-clinical In Vivo Animal Imaging Systems
  • Nikon N-SIM/N-STORM Super-resolution Microscope
  • FUJIFILM VisualSonics Vevo LAZR Multi-modality Imaging Platform
  • Seahorse XFe24 Extracellular Flux Analyzer
  • Malvern MicroCal PEAQ-ITC Automated Ultrasensitive Isothermal Titration Calorimeter
  • Bio-Rad Bio-Plex 200 System
Existing users may access the ULS online booking system to book for these equipment from 1st July, 2017. Prospective users should contact the equipment-in-charge for the procedure to apply for access to the equipment.
 
The ULS is co-organising a seminar with Invitrogen to introduce the recent advancement in cellular analysis. Details of the seminar are as follows:
Title: Recent Advancement in Cell Analysis
Speaker: Dr Raquel Magalhães, Product Manager, Biosciences, Asia Pacific, Invitrogen
Date: 13th June, 2017 (Tuesday)
Time: 3:00 – 4:45 pm
Venue: Y716, Lee Shau Kee Building (Core Y)
 
The ULS is co-organising a seminar with Zeiss to introduce the principles of lightsheet microscopy and how you can utilise the Lightsheet Z.1 system in your research. Details of the seminar are as follows:
Title: Gentle 3D Imaging of Large Biological Specimen – Lightsheet Z.1
Speaker: Mike Lau, Sales and Application Specialist – Microscopes, Carl Zeiss Far East Co., Ltd
Date: 15th May, 2017
Time: 10:00 – 11:00 am
Venue: Y716, Lee Shau Kee Building (Core Y)
 
A charging system for equipment usage will be implemented from 1 May 2017. Please refer to the ULS Charging Scheme and Section 7 of the ULS General User Policy for details.
 
The ULS General User Policy is available for download. Users and supervisors are required to abide to the regulations set out herein.
 
Please be advised that online bookings cannot be modified or cancelled within 24 hours prior to the beginning of a booked session. Requests for changes and cancellations within the 24-hour time window shall be made to the equipment-in-charge and might be subject to a penalty equivalent to 50% of the rate of the whole session.
 
Users of phase 1 equipment are reminded to wave their PolyU staff/student card to sign in and out for a booked session from 5 April, 2017 onward. Please contact the ULS if you encounter any problem with the card lock system.
 
The following phase 1 equipment will be available for online booking from 1 April, 2017:
  • Bruker AmaZon Speed ESI-ion Trap-ETD Mass Spectrometer
  • Bruker UltrafleXtreme MALDI-TOF-TOF Mass Spectrometer
  • Agilent 6460 Liquid Chromatography – Electrospray Ionisation Triple Quadrupole Mass Spectrometer
  • Agilent 6540 Liquid Chromatography – Electrospray Ionisation Quadrupole-Time-of-Flight Mass Spectrometer
  • Leica TCS SP8 MP Multiphoton Microscope
  • Leica TCS SPE Confocal Microscope
  • BD FACSAria III Cell Sorter
  • BD Accuri C6 Flow Cytometer
Registered users may access the ULS online booking system from 27 March, 2017 to book for these equipment from 1 April, 2017.
 
Slides for the ULS introductory session have been uploaded and are available for download.
 
Instructions for registration and online booking are available for download.
 
Two identical introductory sessions for the new online booking system will be held on 10th and 13th March, 2017, from 2:30 to 3:15 pm in Y716 and Y715, respectively. Registration should be made by emailing to uls.notice@polyu.edu.hk and indicating your preferred session.
 
 
Page / Total Page 1