Zeiss Lightsheet Z.1 Selective Plane Illumination Microscope

Description:

Light sheet fluorescence microscopy (LSFM), also termed selective plane illumination microscopy (SPIM), is a planar illumination technique in which fluorescence excitation and detection are decoupled into separate optical pathways. With this novel optical design, illumination using a thin laser light sheet provides an inherent optical sectioning capability without the need to adopt a pinhole, which significantly improves the efficiency of photon collection and imaging speed without compromising image quality. LSFM allows researchers to examine live, intact animal models, ex vivo tissue explants, 3-dimensional cell cultures and organoids, etc., at unprecedented speed with virtually no photobleaching or photocytotoxicity.
 

Specifications:

• Single- or dual-side laser light sheet illumination
• Scanned laser light sheet to produce homogenous illumination across the field-of-view (FOV)
• Pivot scan option to reduce stripes commonly observed in LSFM images
• Multiview imaging to acquire complimentary information from different angles
• Tiling tool to acquire panoramic images covering a substantially larger FOV
• Dedicated sample chambers for live and CLARITY-cleared samples (sample size up to 1×1×2 cm³)
• Detection objectives:
• EC Plan-NEOFLUAR 5×/0.16
• W Plan-APO 20×/1.0 DIC
• W Plan-APO 40×/1.0 DIC
• Clr Plan-NEOFLUAR 20×/1.0 Corr n_d=1.45
• Illumination objectives:
• LSFM 5×/0.1
• LSFM 10×/0.2
• Continuous internal zoom optics: 0.36×-2.5×
• Excitation lasers:
• 405 nm
• 488 nm
• 561 nm
• 638 nm
• Fluorescence filter sets:
• DAPI-Cy3
• DAPI-GFP
• GFP-Cy3
• GFP-DRAQ5
• Universal green-red
• Two high-speed, highly sensitive sCMOS cameras (pco.edge 5.5):
• High-definition (i.e., 2560×2160 pixels)
• High-speed (i.e., 66 fps at 640×640 pixels; 20 fps at 1920×1920 pixels)
• High quantum efficiency (i.e., 60% at peak)
• Extremely low noise (i.e., ~1.0 electron read noise)

Location: Y405

Hourly rate: $178 (internal users); $356 (collaborators); $890 (external users)

Discount for long-duration bookings:

• Applicable to consecutive bookings of 8 hours or longer
• A 50% discount will be applied to any segments that fall within non-office hours (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
• Price adjustments, if any, will be made after the completion of the imaging session
• The ULS reserves the right to interpret these terms

Staff-in-charge: Dr Michael Yuen and Dr Alan Leung

Training: please contact Dr Michael Yuen (email: michael.yf.yuen@polyu.edu.hk / Tel.: 3400 8788) or Dr Alan Leung (email: alan.sl.leung@polyu.edu.hk / Tel.: 3400 8984) for arrangement.