Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging System

Description:

The Nikon Eclipse Ti2-E live-cell imaging system is an inverted, fully motorised, large field-of-view wide-field fluorescence microscope equipped with a collection of state-of-the-art features for live-cell and large-tissue tile imaging.

Specifications:

• Large (i.e., 25 mm) field-of-view (FOV)
• Fly-eye lens design to provide uniform illumination across the whole FOV
• High-precision motorised stage with encoder
• Adaptive auto-focusing system (Nikon Perfect Focus System)
• Real-time deconvolution and deblurring
• Multi-well plate holder and high-content imaging software module for high-throughput imaging and data analysis
• Tile scanning and image stitching to produce high-resolution overview image of large tissue sections
• Environmental chamber with temperature and CO₂ concentration control (Tokai Hit STX series)
• Motorised condenser, objective and fluorescence filter turrets
• Imaging and contrasting modes:
• Bright-field
• Phase contrast
• Differential interference contrast (DIC)
• Wide-field fluorescence
• Multi-wavelength LED illumination system (CoolLED pE-4000) offering 16 selectable wavelengths across 4 channels:
• Channel 1: 365/385/405/435 nm
• Channel 2: 460/470/490/500 nm
• Channel 3: 525/550/580/595 nm
• Channel 4: 635/660/740/770 nm
• Fluorescence filter sets:
• DAPI (460/50)
• FITC (525/50)
• mCherry (630/75)
• Cy5 (690/50)
• DAPI/FITC/TRITC/Cy5 multi-band
• Objectives (details):
• CFI Plan Fluor 4×/0.13 Dry
• CFI Plan Fluor 10×/0.30 Dry
• CFI Super Plan Fluor 20×/0.45 Dry
• CFI Super Plan Fluor 20×/0.70 Dry (on request)
• CFI Super Plan Fluor 40×/0.60 Dry
• CFI Plan Apo 20×/0.75 Dry
• CFI Plan Apo 40×/0.95 Dry (on request)
• CFI Plan Apo 60×/1.40 Oil
• Scientific-grade monochrome CMOS camera (Nikon DS-Qi2):
• Large-format (i.e., 36 × 23.9 mm) and optimised for 25-mm FOV
• High pixel density (i.e., 16.25 megapixel)
• High quantum efficiency (i.e., ~77% at peak)
• Extremely low noise (i.e., ~2.2 electron read noise)
• Electronic cooling system
• High-speed (i.e., 6 fps at full pixel or 45 fps with 3×3 pixel averaging)
• Scientific-grade colour CMOS camera (Nikon DS-Ri2):
• Large-format (i.e., 36 × 23.9 mm) and optimised for 25-mm FOV
• High pixel density (i.e., 16.25 megapixel)
• Excellent colour reproduction
• High-speed (i.e., 6 fps at full pixel or 45 fps with 3×3 pixel averaging)

Location: Y708a

Hourly rate: $49 (internal users); $98 (collaborators); $245 (external users)

Discount for long-duration bookings:

• Applicable to consecutive bookings of 8 hours or longer
• A 50% discount will be applied to any segments that fall within non-office hours (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
• Price adjustments, if any, will be made after the completion of the imaging session
• The ULS reserves the right to interpret these terms

Consumables:

• Marienfeld no. 1.5H 18 mm round coverglass ($148; 100 pcs)
• MatTek 35 mm no. 1.5 glass bottom culture dish ($190; 10 pcs)
• Nunc Lab-Tek II no. 1.5 8-well chambered coverglass ($435; 8 pcs)
• ProLong Diamond antifade mountant ($372; 2 mL)
• ProLong Live antifade reagent ($333; 1 mL)
• EMS 16% paraformaldehyde ($116; 10 mL)

Staff-in-charge: Dr Michael Yuen and Dr Alan Leung

Training: please contact Dr Michael Yuen (email: michael.yf.yuen@polyu.edu.hk / Tel.: 3400 8788) or Dr Alan Leung (email: alan.sl.leung@polyu.edu.hk / Tel.: 3400 8984) for arrangement.