Nikon SMZ1270i Fluorescence Stereomicroscope

Description:

The Nikon SMZ1270i fluorescence stereomicroscope is a parallel-optics and zooming type stereomicroscope offering advanced operability, as well as high magnification, zoom ratio and resolution.

Specifications:

• Parallel-optics and zooming type system
• Objectives that offer long working distances (WDs) and wide viewfields:
• Plan Apo 1x/WF; WD 70 mm
• ED Plan 2×/WF; WD 35 mm
• Highest-in-class zoom ratio of 12.7× (i.e., 0.63-8×)
• "Intelligent nosepiece" to feed objective magnification and zoom ratio information to the camera control unit
• Illumination options:
• Episcopic illumination with double-arm fibre illuminator
• Diascopic bright-field illumination
• Oblique coherent contrast (OCC) illumination with contrast adjustment
• Episcopic fluorescence illumination with multi-wavelength LED illumination system
• White-light LED fibre optic illumination system (CoolLED pE-300^lite) with spectral coverage from ultraviolet to red region
• Fluorescence filter sets:
• DAPI (460/50)
• GFP (535/50)
• RFP (620/60)
• High-definition colour CMOS camera (Nikon DS-Fi3):
• High-definition (i.e., 2880×2048 pixels)
• High-speed (i.e., 15 fps at full pixel or 30 fps with 2×2 pixel averaging)
• Sensitivity selectable from ISO50 to ISO3200
• Camera control unit (Nikon DS-L4):
• Tablet panel display for easy setup and control of the camera
• High-definition display of live and captured images
• Automatic detection of objective magnification and zoom ratio to provide real-time scale information and measurement results
• Direct storage of captured images (TIFF or JPEG) in USB storage devices

Location: Y405

Hourly rate: $19 (internal users); $38 (collaborators); $95 (external users); waived for users who have a concurrent booking of the Zeiss Lightsheet Z.1 Microscope

Staff-in-charge: Dr Michael Yuen and Dr Alan Leung

Training: please contact Dr Michael Yuen (email: michael.yf.yuen@polyu.edu.hk / Tel.: 3400 8788) or Dr Alan Leung (email: alan.sl.leung@polyu.edu.hk / Tel.: 3400 8984) for arrangement.