Description:
The BD FACSAria III cell sorter is built on the solid foundation of patented technologies. The sorter has superior multicolour performance and its ease-of-use has opened the complex world of cell sorting to researchers of various fields and a wider variety of applications. The fluidics and optical systems are precisely integrated to maximise signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve unrivaled sensitivity and resolution.
Specifications:
Optics:
- Lasers: blue (488 nm), red (633 nm) , yellow green (561 nm), violet (405 nm) and near-UV (375 nm)
- Detectors: 2 light scatter detectors and 14 fluorescence detectors with optical filters optimised for the detection of most commonly used fluorochromes
- Violet (405 nm) laser shares the same pinhole with near-UV (375 nm) laser, they cannot be used simultaneously
- Maximum 12 colors can be detected simultaneously
- Trigon/ octagon-shaped pathways of the collection optics to maximise signal detection
- ULS FACSAriaIII Optical Filter Set
Fluidics:
- Pressure-driven: Positive air pressure forces samples through an gel-coupled quartz cuvette flow cell
- Analysis speed: Up to 70,000 events/s
- Shealth and Ethanol filter user bulletin
- Removable 70, 85, 100, and 130-µm nozzle
- Automatic Cell Deposition Unit (ACDU) for single cell sorting or index sorting into 6, 24, 48, 96, and 384-well plates
Location: Y709
Hourly rate: $110 (internal users); $220 (collaborators); $550 (external users)
Consumables: Falcon 5 mL round bottom test tube with cell strainer, $9 each
