Invitrogen Countess II FL Automatic Cell Counter

Description:

The Invitrogen Countess II FL Automatic Cell Counter is a compact system capable of acquiring bright-field and fluorescence images of cell suspensions; and by automatic analysis of the images, allows researchers to instantaneously obtain such information as the cell count, labelling of fluorescent probes, expression of fluorescent proteins, transfection efficiency and cell viability, etc.

Specifications:

• Automated bright-field and fluorescence image acquisition
• LED light cubes:
• GFP (compatible with GFP, FITC, Alexa Fluor 488, SYBR Green, SYTOX Green, etc.)
• RFP (compatible with RFP, DsRed, Rhodamine Red, Alexa Fluor 546/555/568, Cy3, SYTOX Orange, etc.)
• 2.5× objective lens with a final magnification of 50×
• Built-in digital camera with a 5-megapixel sensor
• Automatic focusing system with optional manual focus override
• Compatible chamber slides:
• Countess disposable cell counting chamber slides (Invitrogen #C10228)
• Countess II FL reusable slides (Invitrogen #A25750)
• 2 sample chambers per slide
• 10 µL of cell suspension is required for loading each chamber
• Sample requirements:
• Cell size: ~4 to 60 µm in diameter
• Cell concentration: ~1 × 10⁴ to 1 × 10⁷ cells/mL
• Non-motile cells
• Built-in dilution calculator
• USB port for exporting images, counting data, graphs, etc.

Location: Y709

Hourly rate: no charge

Consumables:

• Disposable cell counting chamber slide ($16)
• Box of 50 disposable cell counting chamber slides ($800; includes a 1-mL vial of 0.4% trypan blue)

Staff-in-charge: Dr Michael Yuen

Training: please contact Dr Michael Yuen (email: michael.yf.yuen@polyu.edu.hk / Tel.: 3400 8788) for arrangement.