Sciex 6500 + Liquid chromatography/Electrospray Ionization - QTrap Mass Spectrometer

Description:

The Sciex 6500+ Liquid chromatography/Electrospray Ionization - QTrap Mass Spectrometer (LC/ESI-Qtrap MS) allows quantification of target analytes in complex biological and chemical samples with high sensitivity (typically low ppb level) and specificity. It also permits qualitative confirmation of compounds through high quality product ion scan and MS³ scan. It is equipped with an ion-mobility device for separation of isomeric compounds and reduction in background interference.

Specifications:

Liquid Chromatography: ExionLC AD series (UHPLC)

• 4 solvent channels with individual pumps (max pressure: 1300 bar)
• Autosampler with thermostat
• Column oven
• Online solid-phase extraction (SPE) is allowed

Mass Spectrometer: 6500+ Qtrap

• Ionization modes: Electrospray ionization (ESI), Atmospheric-pressure chemical ionization (APCI)
• Scan modes: MS scan, product ion scan, multiple reaction monitoring (MRM), selected ion monitoring (SIM), neutral loss scan, precursor ion scan, MS³ scan
• Mass range: m/z 5 – 2,000
• Dynamic range: six orders of magnitude
• SelexION ion-mobility device

Software:

• Analyst, SCIEX-OS

Applications:

• Pharmacokinetics
• Quantification of various molecules (metabolites, lipids, peptides, proteins, etc) in biological samples
• Food analysis
• Environmental analysis
• Natural product analysis

Location: W711

Hourly rate: $79 (internal users); $158 (collaborators); $395 (external users)

Staff-in-charge: Dr Carol Tsang and Dr So Pui-Kin